The Three Lions boss will be nervously watching club football over the next few days, hoping that his plans will not be further disrupted by injury problems in the build-up to the tournament which begins on November 20.
Reece James is a major doubt after the Chelsea star picked up a knee injury that is expected to keep him out for eight weeks, while Manchester City duo Kyle Walker and Kalvin Phillips are both in a race to prove their fitness after undergoing surgery, with Ben Chilwell out.
There are also those on the fringes of the England squad who will be looking to make their case for a place on the plane with a strong run of performances in the coming weeks, with Ivan Toney insisting he is “ready” to perform for the national side.
England face Iran first up in Group B, before matches against the USA and Wales as Southgate’s men look to progress through to the knockout stages in routine fashion.
As was the case at Euro 2020, 26-man squads will be selected for the tournament after FIFA approved the change earlier this year.
When do England name World Cup squad?
Southgate is expected to select his World Cup squad on Thursday, November 10 at 1pm GMT.
The England boss is still able to make changes after that, up until the final FIFA deadline of Sunday, November 13 when the squad must be submitted officially.
The Three Lions get their World Cup campaign underway eight days later when they face Iran on November 21.